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Image Search Results
Journal: PeerJ
Article Title: Quality assessment of a serum and xenofree medium for the expansion of human GMP-grade mesenchymal stromal cells
doi: 10.7717/peerj.13391
Figure Lengend Snippet: Supernatant of THP-1 cells co-cultured with MSC at P1 and P2 was assayed for TNF-α and IL-1ra secretion. The cytokine concentration is normalized by the one measured in the LPS-treated THP-1 monoculture . Data are presented as median ± interquartile range with n = 7 for TNF- α secretion by BM-MSC, n = 6 for IL-1ra secretion by BM-MSC, n = 5 for TNF- α secretion by Ad-MSC and n = 4 for IL-1ra secretion by Ad-MSC. Friedman test was used to compare the effect of MSC expanded in the 2 media (hCPL and SXFM) to THP-1 stimulated by LPS, ns = non-significant, ∗ p < 0.05 and ∗∗ p < 0.01.
Article Snippet: As highly encouraged by the regulators to have a backup media in all Good Manufacturing Practices (GMP) processes (Guideline on human cell-based medicinal products, EMEA/CHMP/410869/2006), the aim of this study was to validate the use of a
Techniques: Cell Culture, Concentration Assay
Journal: International Journal of Molecular Sciences
Article Title: Neuronal Differentiation and Exosome Profiling of Dental Pulp Stem Cells: Unveiling Their Potential for Nerve Repair
doi: 10.3390/ijms26199723
Figure Lengend Snippet: ∆Ct values for the different genes under study in hDPSCs, undifferentiated and after neurogenic differentiation. Higher ∆Ct values represent lower expression. Results are presented as mean ± SEM.
Article Snippet: Media was removed from all the wells, and the
Techniques: Expressing